Estimation of genetic diversity in the germplasm of Brassica Campestris (L.) Using morphological and biochemical markers
A total of 60 genotypes of Brassica campestris (L) germplasm were acquired from National Research Centre (NARC) Islamabad, Pakistan for the evaluation of genetic diversity using the morphological characters and SDS-PAGE analysis. These genotypes were grown-up in Plant Garden Department of plant sciences, University of Malakand, Khyber Pukhtunkhwa, Pakistan. For the assessment of genetic diversity total of 18 morphological traits were scored and their phylogenetic relationship were plot through cluster diagram. For the estimation of genetic diversity, we used the most influential technique SDS PAGE. We observed six loci (bands) from the collected genotypes; locus-1 (band-1) contained 100% bands of protein and obvious as monomorphic locus while loci i.e. 2,3,4,5 and 6 showed 28%, 42%, 36%, 56% and 25%, respectively variation and were polymorphic. The inter species locus influence toward genetic assortment (LCTGA) was 83.33% in the attained genotypes. Profiling through SDS PAGE based on two-way cluster plotting resolved effectively the collected genotype into 4 cluster. We consider that this work provides a baseline for the understanding genetic diversity in the common crops used in Pakistan and warrants further investigation in this newly evolving field of study.